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Image Search Results
Journal: Journal of Traditional Chinese Medical Sciences
Article Title: Exploring the molecular mechanism of Epimedium brevicornu Maxim. in treating breast cancer via network pharmacology and in vitro experiments
doi: 10.1016/j.jtcms.2024.03.005
Figure Lengend Snippet: Fig. 12. Effects of drug treatment on the expression of (A) Bax and PCNA, and (B) TBK1/NAK via western blot analysis. MDA-MB-231 cells were treated with Anhy or Iso for 24 h at the indicated concentration (20 mmol/L). Notes: Con: Control; DMSO: dimethylsulfoxide; Iso: isoliquiritigenin; Anhy: b-anhydroicaritin. Con: MDA-MB-231 cells in complete culture medium containing 1% fetal bovine serum. DMSO was used for the dissolution of Anhy and Iso. The DMSO group was included in the analysis to check for solvent interference in the results. To ensure equal loading of protein, b-actin was used as a loading control. The respective bar graphs are presented as densitometry analysis as mean (standard deviation) of experiments. *P < .05 and **P < .01 vs. the Con group.
Article Snippet: The primary antibodies: TBK1 (1:2000, Cat# 3013S), Bax (1:1000, Cat# 5023T), and
Techniques: Expressing, Western Blot, Concentration Assay, Control, Dissolution, Solvent, Standard Deviation